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Primer Design Designing the correct primer sequence can have a large impact on the success of a PCR reaction. Through our automated proprietary primer design software we can design and deliver the optimal set of primers to ensure success. Construct Design Our experience indicates that truncating or mutating a protein sequence by as little as one amino acid can be the difference between soluble and insoluble protein expression results. Using our proprietary bioinformatics techniques we can suggest DNA constructs that increase probability of obtaining successfully expressed protein. Gene Synthesis Gene Synthesis is a powerful new technology for delivering difficult to isolate genes. Gene synthesis is particularly useful when applying domain parsing techniques, mutagenesis techniques, or when working with difficult to isolate eukaryotic cDNA pools. Nexomics Biosciences offers Gene Synthesis as an alternative to traditional molecular cloning. |
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Site Directed Mutagenesis Site directed mutagenesis is used in molecular biology to evaluate functionality as well as structural biology to promote order protein as well as crystallization. Using our protein production platform we can engineer specific mutations into target protein sequences. HTP Expression Vector Construction Molecular cloning is the process of obtaining multiple copies of a desired DNA sequence. Our most frequent usage of molecular cloning is to amplify a specific gene out of host DNA. Nexomics Biosciences utilizes a proprietary high-throughput 96-well robotic cloning platform to rapidly provide amplified DNA. HTP Expression/Solublity Screening Prior to committing resources toward large scale fermentation it is suggested to test solubility and expression on a smaller micro liter scale. As part of our automated high throughput molecular cloning platform we are able to conduct small scale test expression / solubility studies. The results of this analysis can be used to guide decision making for large scale multi-liter fermentation. Large Scale Expression Nexomics Biosciences offers large scale multi-liter fermentation. We support a wide range of expression systems and media types. |
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Purification Nexomics Biosciences uses an automated two step process using Nickel column purification followed by analytical gel purification to ensure the highest purity protein is obtained. We are then able to confirm molecular weight using mass spectroscopy and Caliper technology. Alternative purifications techniques are employed for difficult proteins such as various different purification tags as well as refolding. |
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Buffer Optimization We have found that buffer conditions can have a significant impact on protein solubility. Nexomics Biosciences has developed a high throughput buffer optimization platform to screen for optimal buffer conditions. Our platform utilizes Nuclear Magnetic Resonance (NMR) technology as well as an automated sample changer. |
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Mass Spectroscopy Mass spectroscopy is a powerful technique for experimentally calculating molecular weight. Nexomics Biosciences makes use of MALDI-TOF mass spectroscopy as a quality control check for all protein production projects. The technology can also be used to measure regions of disorder within a protein. |
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Analytical Gel Filtration followed by Static/Dynamic Light Scattering This powerful technique is used to experimentally calculate protein oligmeric state. Often for structural biology studies it is necessary to be sure of the primary oligmeric state of the native protein. Nexomics Biosciences has extensive expertise in measuring target protein oligmeric state by combining gel filtration with light scattering techniques. Functional Assay Development Let Nexomics Biosciences assist you in planning and developing a functional assay for your biological system of interest. While every system is different we have experience developing assays across many different fields of biology. |
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