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Protein Sequence Analysis
A detailed understanding of a target protein’s amino acid composition, predicted secondary structure, predicted thermal stability and evolutionary history can often give insight toward protein function as well as an overall feasibility for expression, purification and ultimately structure determination. Nexomics Biosciences offers a standard bioinformatics analysis of both DNA and Protein sequences of interest which often are used to complement our Protein and Structural Biology Services.

Construct Optimization / Domain Parsing
Often it is possible to express difficult proteins by engineering the protein sequence to remove regions of disorder, or other features which may promote instability. Our proprietary techniques allow us to suggest the optimal protein sequence to promote thermodynamic stability while leaving the native protein’s three dimensional structure and functionality unperturbed.

Homology Modeling
Homology modeling is a form of protein structure prediction where a three dimensional protein structure is modeled from an existing amino acid sequence of known structure known as the template. The closer the unknown amino acid sequence is to that of the template, the higher confidence one can have in an accurately homology modeled three dimensional structure. Homology models are useful for pathway modeling, predictive protein-protein / protein-ligand interactive simulations and in general as a guide in the protein structure determination process. Nexomics Biosciences offers homology modeling using our proprietary modeling structure validation software.

DNA Codon Optimization
It is well established that different protein expression systems contain varying abundances of specific tRNAs. Low expression levels due to tRNA abundance can often be overcome by engineering target DNA to contain a higher level of native tRNA needing codons. Furthermore, this engineering can often be done in such a way as to have no effect on the final protein sequence. Our proprietary methods allow us to suggest optimized DNA constructs which often promote increased expression in non-native expression systems.